cebpd antibody  (Abmart Inc)

Journal: Journal of Inflammation Research

Article Title: Identification and Validation of Lipid Metabolism-Related Biomarkers GPD1 and CEBPD in Metabolic Dysfunction-Associated Steatohepatitis

doi: 10.2147/JIR.S524204

Figure Lengend Snippet: Diagnostic Performance and Clinical Relevance of CEBPD and GPD1 in MASH Patients. ( A ) Comparison of risk distributions between patients with MASH and healthy controls in the test set. ( B ) ROC curves assessed the model’s diagnostic performance in the test set. ROC curves assessed the diagnostic performance of CEBPD ( C ) and GPD1 ( D ) in the train set. ( E ) Comparison of CEBPD and GPD1 expression levels between patients with MASH and HC in the test set. ROC curves evaluated the diagnostic performance of CEBPD ( F ) and GPD1 ( G ) in the test set. ( H and I ) The association between the expression level of CEBPD and GPD1 and the fibrosis stage. * p < 0.05, *** p < 0.001, **** p < 0.0001, ns not significant ( p ≥ 0.05).

Article Snippet: The GPD1 antibody was obtained from Proteintech, while the CEBPD antibody was sourced from Abmart.

Techniques: Diagnostic Assay, Comparison, Expressing

Journal: Journal of Inflammation Research

Article Title: Identification and Validation of Lipid Metabolism-Related Biomarkers GPD1 and CEBPD in Metabolic Dysfunction-Associated Steatohepatitis

doi: 10.2147/JIR.S524204

Figure Lengend Snippet: Immune Cell Profiling and Correlation with CEBPD and GPD1 Expression in MASH. ( A ) Heatmap showing correlations between 28 immune cell types in MerCorhort. ( B ) Heatmap showing the infiltrating enrichment of 28 immune cell types in MerCorhort. ( C ) Boxplot depicting differences in immune cell infiltration between patients with MASH and healthy controls. ( D ) Heatmap showing the correlation between CEBPD and GPD1 expression and immune cells in MASH. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns not significant ( p ≥ 0.05).

Article Snippet: The GPD1 antibody was obtained from Proteintech, while the CEBPD antibody was sourced from Abmart.

Techniques: Expressing

Journal: Journal of Inflammation Research

Article Title: Identification and Validation of Lipid Metabolism-Related Biomarkers GPD1 and CEBPD in Metabolic Dysfunction-Associated Steatohepatitis

doi: 10.2147/JIR.S524204

Figure Lengend Snippet: Validation of GPD1 and CEBPD in a MASH Animal Model. ( A ) IHC staining of normal and MASH liver tissues, n = 6, 400×, scale bar 100 μm. ( B ) AOD values for GPD1 in CON and MASH groups. ( C ) AOD values for CEBPD in CON and MASH groups. ( D ) WB analysis of normal and MASH liver tissues, n = 3. ( E ) Relative gray value measurements for GPD1 in CON and MASH groups. ( F ) Relative gray value measurements for CEBPD in CON and MASH groups. *** p ≤ 0.001.**** p ≤ 0.0001.

Article Snippet: The GPD1 antibody was obtained from Proteintech, while the CEBPD antibody was sourced from Abmart.

Techniques: Biomarker Discovery, Animal Model, Immunohistochemistry

Journal: Cell Death Discovery

Article Title: Epithelial CEBPD activates fibronectin and enhances macrophage adhesion in renal ischemia-reperfusion injury

doi: 10.1038/s41420-024-02082-4

Figure Lengend Snippet: A Cebpd expression in U-IRI mice. Tubular epithelial cells were recognized with E-cadherin antibodies (green); CEBPD was recognized with specific CEBPD antibodies (red). The dead epithelial cells are marked with yellow arrows. Images were taken with a fluorescence microscope at a magnification of 200×. ( n = 3 for each group). B Cebpd is mainly induced in the kidney epithelial cells of U-IRI mice following the quantification of fluorescence intensity using ImageJ software (Student’s t test; * P < 0.05, ** P < 0.01, *** P < 0.001).

Article Snippet: Subsequently, the slides were incubated with specific antibodies targeting CEBPD (sc‐365546; Santa Cruz Biotechnology), F4/80 (sc-377009; Santa Cruz Biotechnology), FN-1 (#15613-1-AP; Proteintech), and E-cadherin (GTX100443; GeneTex), each antibody was diluted at a ratio of 1:200.

Techniques: Expressing, Fluorescence, Microscopy, Software

Journal: Cell Death Discovery

Article Title: Epithelial CEBPD activates fibronectin and enhances macrophage adhesion in renal ischemia-reperfusion injury

doi: 10.1038/s41420-024-02082-4

Figure Lengend Snippet: A FN-1 levels are attenuated in the kidneys of Cebpd -deficient mice following U-IRI administration. FN-1 expression was examined via Western blotting and quantified by statistical analysis. (one-way ANOVA; * P < 0.05, ** P < 0.01, *** P < 0.001). B The number of macrophages surrounding epithelial cells was attenuated in the kidneys of U-IRI Cebpd -deficient mice. The macrophages were labeled with F4/80 (red) and FN-1 was detected using a specific anti-FN-1 antibody (green). The images were taken with a fluorescence microscope and magnified at 200×. C The fluorescence intensity of colocalized F4/80 and FN-1 signals in the kidneys of U-IRI mice was quantified by ImageJ software ( n = 6 for each group) (one-way ANOVA; * P < 0.05, ** P < 0.01, *** P < 0.001).

Article Snippet: Subsequently, the slides were incubated with specific antibodies targeting CEBPD (sc‐365546; Santa Cruz Biotechnology), F4/80 (sc-377009; Santa Cruz Biotechnology), FN-1 (#15613-1-AP; Proteintech), and E-cadherin (GTX100443; GeneTex), each antibody was diluted at a ratio of 1:200.

Techniques: Expressing, Western Blot, Labeling, Fluorescence, Microscopy, Software

Journal: Cell Death Discovery

Article Title: Epithelial CEBPD activates fibronectin and enhances macrophage adhesion in renal ischemia-reperfusion injury

doi: 10.1038/s41420-024-02082-4

Figure Lengend Snippet: A Exogenous CEBPD activates FN-1 transcription in HK-2 cells. A RT‒qPCR assay was performed with total RNA from control and CEBPD-expressing vector transfectants. (Student’s t test; * P < 0.05, ** P < 0.01, *** P < 0.001). B Exogenous CEBPD activates the FN-1 reporter in HK-2 cells. A reporter assay was performed with cell lysates from the FN-1 reporter and control or CEBPD-expressing vector transfectants. (Student’s t test; * P < 0.05, ** P < 0.01, *** P < 0.001). C The inactivation of CEBPD attenuates hypoxia-induced FN-1 reporter activity in HK-2 cells. A reporter assay was performed with cell lysates from FN-1 reporter and lentiviral shcontrol (shCtl)- or shCEBPD (shCD)-infected transfectants. (one-way ANOVA; * P < 0.05, ** P < 0.01, *** P < 0.001). D CEBPD (CD) directly binds to the FN-1 gene promoter and this binding is responsive to hypoxia in HK-2 cells. A ChIP-PCR assay was performed with the indicated antibody-pull down formaldehyde-treated HK-2 cell lysates under normoxic (N) or hypoxic (H) conditions (Student’s t test; * P < 0.05, ** P < 0.01, *** P < 0.001).

Article Snippet: Subsequently, the slides were incubated with specific antibodies targeting CEBPD (sc‐365546; Santa Cruz Biotechnology), F4/80 (sc-377009; Santa Cruz Biotechnology), FN-1 (#15613-1-AP; Proteintech), and E-cadherin (GTX100443; GeneTex), each antibody was diluted at a ratio of 1:200.

Techniques: Control, Expressing, Plasmid Preparation, Reporter Assay, Activity Assay, Infection, Binding Assay